In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels

Samuel S. Hinman, Yuli Wang, Raehyun Kim, Nancy L. Allbritton

Published: 2020-12-08 DOI: 10.1038/s41596-020-00419-8

intestinal epithelia

hydrogel scaffold

stem cells

crypt formation

growth factor gradients

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Supplementary information

Supplementary Information

Supplementary Figs. 1–3 and Supplementary Table 1.

Reporting Summary

Supplementary Data 1

Sheet 1: collagen neutralization volumes; Sheet 2: subcultivation ratios

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues

Decoding personal biotic and abiotic airborne exposome

A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys

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Biomimetic tail-to-head terpene cyclizations using the resorcin[4]arene capsule catalyst

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Scanorama: integrating large and diverse single-cell transcriptomic datasets

Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Data 1

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