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    Protocol for Bulk Water Enrichment for the Isolation of Salmonella from Surface Water

    Autumn L Kraft, Manan Sharma, James E Wells, Abasiofiok Mark Ibekwe, NARMS EWG, Jonathan G Frye

    Published: 2022-06-15 DOI: 10.17504/protocols.io.ewov1n3xygr2/v1
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    Abstract

    This protocol details the bulk water enrichment for the isolation of Salmonella from surface water.

    Attachments

    411-892.docx

    Steps

    Protocol

    1.

    Take 1 of the water sample bottles (containing 1L of surface water) and carefully transfer water into a sterile 2 L Pyrex bottle.

    2.

    If needed, add control strain to water sample by gently tipping vial over the opening. Close bottle tightly and thoroughly mix sample.

    3.

    Aseptically measure 250mL of 5X UPB and pour into 2 L bottle (making final concentration of 1X UPB)

    4.

    Secure lid and mix thoroughly before incubating at 37°C for 18-24 h.

    Note
    For next steps, refer to “Selective Enrichment Protocol for Salmonella Isolation from Surface Water.”

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